Real-time PCR Materials:

All required materials are listed in the downloadable Recipe Spreadsheet.  Briefly, these include:

1. cDNA of your sample
2. Master mix
3. Real-time PCR machine

All PCRs in our lab are run on Applied Biosystems real-time PCR machines.  A publicly available real-time PCR machine (7900 HT) is located in UMDNJ's sequencing facility. 

I have compared SYBR green master mix ingredients from Takara, Applied Biosystems, and Qiagen.  Each of these provide similar results with identical samples.  Our lab uses only Applied Biosystems reagents for PCR reactions. 

For more details regarding required reagents, please see the Recipe Spreadsheet.